Overview

  • Product nameAnti-RanBP9 antibody
    See all RanBP9 primary antibodies
  • Description
    Rabbit polyclonal to RanBP9
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Chimpanzee, Opossum
  • Immunogen

    Recombinant fusion protein: PHGDSALNEQ EKELQRRLKR LYPAVDEQET PLPRSWSPKD KFSYIGLSQN NLRVHYKGHG KTPKDAASVR ATHPIPAACG IYYFEVKIVS KGRDGY, corresponding to amino acids 133-229 of Human RanBP9

  • Positive control
    • Crude extracts of HeLa and U2OS cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
  • Tissue specificityUbiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
  • Sequence similaritiesBelongs to the RANBP9/10 family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 CTLH domain.
    Contains 1 LisH domain.
  • DomainThe SPRY domain mediates the interaction with MET, AR, and CDC2L1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
    Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
  • Cellular localizationNucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell antigen receptor Ig beta associated protein 1 antibody
    • BPM 90 antibody
    • BPM L antibody
    • BPM-L antibody
    • BPM90 antibody
    • BPML antibody
    • IBAP 1 antibody
    • Imp 9 antibody
    • Importin 9 antibody
    • Novel centrosomal protein RanBPM antibody
    • RAN binding protein 9 antibody
    • Ran binding protein centrosomal antibody
    • Ran Binding Protein in the Microtubule organizing center antibody
    • Ran binding protein M antibody
    • Ran BP9 antibody
    • Ran-binding protein 9 antibody
    • Ran-binding protein M antibody
    • RANB9_HUMAN antibody
    • RanBP 7 antibody
    • RANBP 9 antibody
    • RanBP7 antibody
    • RanBP9 antibody
    • RanBPM antibody
    see all

Anti-RanBP9 antibody images

  • All lanes : Anti-RanBP9 antibody (ab64275) at 1/2000 dilution

    Lane 1 : Crude extracts of HeLa cells
    Lane 2 : Crude extracts of U2OS cells.


    Predicted band size : 78 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64275, 1/1000) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab64275 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64275, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-RanBP9 antibody (ab64275)

ab64275 has not yet been referenced specifically in any publications.

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