Recombinant Anti-RanBP9 antibody [EPR9920(B)] (ab140627)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9920(B)] to RanBP9
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RanBP9 antibody [EPR9920(B)]
See all RanBP9 primary antibodies -
Description
Rabbit monoclonal [EPR9920(B)] to RanBP9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human RanBP9 aa 100-200. The exact sequence is proprietary.
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Positive control
- HeLa, fetal brain and JAR lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9920(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
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ICC/IF | (2) |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA. -
Tissue specificity
Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells. -
Sequence similarities
Belongs to the RANBP9/10 family.
Contains 1 B30.2/SPRY domain.
Contains 1 CTLH domain.
Contains 1 LisH domain. -
Domain
The SPRY domain mediates the interaction with MET, AR, and CDC2L1. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11. -
Cellular localization
Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 10048 Human
- Entrez Gene: 56705 Mouse
- Entrez Gene: 364686 Rat
- Omim: 603854 Human
- SwissProt: Q96S59 Human
- SwissProt: P69566 Mouse
- Unigene: 708182 Human
- Unigene: 148781 Mouse
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Alternative names
- B cell antigen receptor Ig beta associated protein 1 antibody
- BPM 90 antibody
- BPM L antibody
see all
Images
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All lanes : Anti-RanBP9 antibody [EPR9920(B)] (ab140627) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa -
Immunofluorescent staining of HeLa cells labelling RanBP9 with ab140627 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab140627 has been referenced in 2 publications.
- Soliman SHA et al. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Sci Rep 10:7138 (2020). PubMed: 32346083
- Bao J et al. RAN-binding protein 9 is involved in alternative splicing and is critical for male germ cell development and male fertility. PLoS Genet 10:e1004825 (2014). PubMed: 25474150