Overview

  • Product nameAnti-RanGAP1 antibody
    See all RanGAP1 primary antibodies
  • Description
    Rabbit polyclonal to RanGAP1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 400-450 of Human RanGAP1 (NP_002874.1).

  • Positive control
    • Human Breast Carcinoma, Human Colon Carcinoma, Human Ovarian Carcinoma, Human Prostate Carcinoma, Human Testicular Seminoma

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab105201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.

Target

  • FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
  • Tissue specificityHighly expressed in brain, thymus and testis.
  • Sequence similaritiesBelongs to the RNA1 family.
    Contains 6 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
    Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
  • Cellular localizationCytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fug 1 antibody
    • Fug1 antibody
    • GTPase-activating protein, RAN, 1 antibody
    • KIAA1835 antibody
    • MGC20266 antibody
    • OTTHUMP00000028918 antibody
    • OTTHUMP00000198755 antibody
    • OTTHUMP00000198756 antibody
    • OTTHUMP00000198757 antibody
    • OTTHUMP00000198758 antibody
    • RAGP1_HUMAN antibody
    • Ran 1 antibody
    • RAN GTPase activating protein 1 antibody
    • Ran GTPase-activating protein 1 antibody
    • Ran1 antibody
    • RANGAP 1 antibody
    • RANGAP antibody
    • RanGAP1 antibody
    • SD antibody
    • Segregation distorter homolog antibody
    • Segregation distortion antibody
    see all

Anti-RanGAP1 antibody images

  • Staining of Human RanGAP1 in a Formalin/PFA-fixed paraffin-embedded section of Human Colon Carcinoma using ab105201 at a dilution of 1/250. Detection utilised DAB staining.

References for Anti-RanGAP1 antibody (ab105201)

ab105201 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"