Overview

  • Product nameAnti-RanGAP1 antibody
    See all RanGAP1 primary antibodies
  • Description
    Rabbit polyclonal to RanGAP1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide mixture: CHWSDMFTGRLRPEI (amino acids 82-96) and CPSPEKLVRMGPRRSA (amino acids 434-448) from Xenopus laevis

  • General notes


    Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, Whole Serum
  • PurityWhole antiserum
  • Primary antibody notesRan is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa.

Target

  • FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
  • Tissue specificityHighly expressed in brain, thymus and testis.
  • Sequence similaritiesBelongs to the RNA1 family.
    Contains 6 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
    Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
  • Cellular localizationCytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fug 1 antibody
    • Fug1 antibody
    • GTPase-activating protein, RAN, 1 antibody
    • KIAA1835 antibody
    • MGC20266 antibody
    • OTTHUMP00000028918 antibody
    • OTTHUMP00000198755 antibody
    • OTTHUMP00000198756 antibody
    • OTTHUMP00000198757 antibody
    • OTTHUMP00000198758 antibody
    • RAGP1_HUMAN antibody
    • Ran 1 antibody
    • RAN GTPase activating protein 1 antibody
    • Ran GTPase-activating protein 1 antibody
    • Ran1 antibody
    • RANGAP 1 antibody
    • RANGAP antibody
    • RanGAP1 antibody
    • SD antibody
    • Segregation distorter homolog antibody
    • Segregation distortion antibody
    see all

Anti-RanGAP1 antibody images

  • ab4784 staining RanGAP1 on skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) 

  • ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure).

    NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa is likely to be a small amount of non-SUMOylated protein.

    ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure).

    NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa&#

  • ab4784 (1/50) staining RanGap1 in mouse NIH-3T3 cells (green). Cells were fixed in methanol (-20oC/ 6 mins, followed by 3 washes in PBS) and DAPI was used as counterstain in order to highlight the nucleus (blue);

References for Anti-RanGAP1 antibody (ab4784)

This product has been referenced in:

See all 3 Publications for this product

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