Overview

  • Product nameAnti-RanGAP1 antibody
    See all RanGAP1 primary antibodies
  • Description
    Mouse polyclonal to RanGAP1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human RanGAP1, amino acids 1-587 (NP_002874.1)

  • Positive control
    • Jurkat cell lysate, RanGAP1 transfected 293T cell lysate, human placenta tissue and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
  • Tissue specificityHighly expressed in brain, thymus and testis.
  • Sequence similaritiesBelongs to the RNA1 family.
    Contains 6 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
    Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
  • Cellular localizationCytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fug 1 antibody
    • Fug1 antibody
    • GTPase-activating protein, RAN, 1 antibody
    • KIAA1835 antibody
    • MGC20266 antibody
    • OTTHUMP00000028918 antibody
    • OTTHUMP00000198755 antibody
    • OTTHUMP00000198756 antibody
    • OTTHUMP00000198757 antibody
    • OTTHUMP00000198758 antibody
    • RAGP1_HUMAN antibody
    • Ran 1 antibody
    • RAN GTPase activating protein 1 antibody
    • Ran GTPase-activating protein 1 antibody
    • Ran1 antibody
    • RANGAP 1 antibody
    • RANGAP antibody
    • RanGAP1 antibody
    • SD antibody
    • Segregation distorter homolog antibody
    • Segregation distortion antibody
    see all

Anti-RanGAP1 antibody images

  • Anti-RanGAP1 antibody (ab88682) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-RanGAP1 antibody (ab88682) at 1 µg/ml

    Lane 1 : RanGAP1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • ab88682 at 3µg/ml staining RanGAP1 in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
  • ab88682 at 10µg/ml staining RanGAP1 in HeLa cells by Immunofluorescence.

References for Anti-RanGAP1 antibody (ab88682)

ab88682 has not yet been referenced specifically in any publications.

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