Recombinant full length Human RanGAP1 produced in HEK293T cells (NP_002874).
HEK293T cell lysate transfected with pCMV6-ENTRY RanGAP1 cDNA; HEK293T cells transfected with RanGAP1 overexpress plasmid; Human liver, prostate and Human ovary adenocarcinoma tissues; HeLa and Jurkat cells; HepG2, HeLa, Jurkat, HT29, A549, MDCK, PC12 and MCF7 cell extracts.
The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificityHighly expressed in brain, thymus and testis.
Sequence similaritiesBelongs to the RNA1 family. Contains 6 LRR (leucine-rich) repeats.
Post-translational modificationsPhosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localizationCytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
ab119092 at 1/100 dilution staining RanGAP1 in HEK293T cells transfected with either pCMV6-ENTRY RanGAP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and analysed by Flow Cytometry.