RanGAP1 overexpression 293T lysate (whole cell) (ab94064)

Overview

  • Product name
    RanGAP1 overexpression 293T lysate (whole cell)
  • Description
    RanGAP1 overexpression lysate
  • General notes
    ab94064 is a 293T cell transfected lysate in which Human RanGAP1 has been transiently over-expressed using a pCMV-RanGAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. PTM: Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. Similarity: Belongs to the RNA1 family. Contains 6 LRR (leucine-rich) repeats. Tissue specificity: Highly expressed in brain, thymus and testis.

Applications

Our Abpromise guarantee covers the use of ab94064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94064 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RanGAP1 antibody (ab88682) at 1/500 dilution

    Lane 1 : RanGAP1 293T Transfected Lysate - Positive Control (ab94064)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated, at 1/2500 dilution

References

ab94064 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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