Anti-RANK antibody [64C1385] (ab13918)

Overview

  • Product nameAnti-RANK antibody [64C1385]
    See all RANK primary antibodies
  • Description
    Mouse monoclonal [64C1385] to RANK
  • Tested applicationsSuitable for: IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment containing the extracellular domain of RANK (Human).

  • Positive control
    • Activated T cells or RAW (a mouse macrophage monocyte).

Properties

Applications

Our Abpromise guarantee covers the use of ab13918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 97 kDa.
Flow Cyt Use 5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • Tissue specificityUbiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • Involvement in diseaseDefects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 265 antibody
    • CD265 antibody
    • FEO antibody
    • LOH18CR1 antibody
    • Loss of heterozygosity 18 chromosomal region 1 antibody
    • mRANK antibody
    • ODFR antibody
    • OFE antibody
    • OPTB7 antibody
    • Osteoclast differentiation factor receptor antibody
    • OSTS antibody
    • Paget disease of bone 2 antibody
    • PDB 2 antibody
    • PDB2 antibody
    • RANK antibody
    • Receptor activator of NF KB antibody
    • Receptor activator of NF-KB antibody
    • receptor activator of nuclear factor kappa B antibody
    • TNFRSF11A antibody
    • TNR11_HUMAN antibody
    • TRANCER antibody
    • Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody
    • Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody
    • Tumor necrosis factor receptor superfamily member 11A antibody
    see all

Anti-RANK antibody [64C1385] images

  • Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.

  • IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-RANK antibody [64C1385] (ab13918)

ab13918 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step None
Sample Human Tissue sections (skin)
Specification skin
Permeabilization Yes - saponine
Fixative Formaldehyde
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Verified customer

Submitted Mar 24 2014

Thank you for your enquiry.

All tested applications and species covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Therefore, I can c...

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Thank you for your response.

The identities of ab13918 with canine were 81%, with 89% positives and some gaps. This is generally the absolute limit for what we consider to be effective. I chose to recommend ab22106 because it was created wit...

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Thank you for your reply. I am pleased to provide the following information: We have two RANK antibodies tested flow cytometry, human and mouse (not IHC or rat): ab45092 RANK antibody [9A725] (Phycoerythrin) Mouse monoclonal Tested applicat...

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Thank you for your email. I have instructed our accounting department to issue a refund for your order for ab13918. If you have any questions regarding the refund, please contact accounts@abcam.com If I can be of additional assistance, just let me k...

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Thank you for your email and patience. I enquired with the originator of this antibody and evidently RANK is a tricky protein to detect and depending upon how the RAW cells were cultured, you may or may not see a signal. However, I would expect that wi...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab13918 in Western blotting. I do need a few more details in order to trouble-shoot this: How much protein did you load? Did you use reducing conditions? How...

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