Anti-RANK antibody [9A725] (Phycoerythrin) (ab45092)

Overview

  • Product nameAnti-RANK antibody [9A725] (Phycoerythrin)
    See all RANK primary antibodies
  • Description
    Mouse monoclonal [9A725] to RANK (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A fusion protein containing amino acid residues 326-616 of human RANK.

  • EpitopeThis antibody recognizes an epitope located between amino acid residues 330-427 of human RANK.
  • Positive control
    • RAW cells

Properties

Applications

Our Abpromise guarantee covers the use of ab45092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-2µg for 106 cells. (intracellular).



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • Tissue specificityUbiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • Involvement in diseaseDefects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 265 antibody
    • CD265 antibody
    • FEO antibody
    • LOH18CR1 antibody
    • Loss of heterozygosity 18 chromosomal region 1 antibody
    • mRANK antibody
    • ODFR antibody
    • OFE antibody
    • OPTB7 antibody
    • Osteoclast differentiation factor receptor antibody
    • OSTS antibody
    • Paget disease of bone 2 antibody
    • PDB 2 antibody
    • PDB2 antibody
    • RANK antibody
    • Receptor activator of NF KB antibody
    • Receptor activator of NF-KB antibody
    • receptor activator of nuclear factor kappa B antibody
    • TNFRSF11A antibody
    • TNR11_HUMAN antibody
    • TRANCER antibody
    • Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody
    • Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody
    • Tumor necrosis factor receptor superfamily member 11A antibody
    see all

Anti-RANK antibody [9A725] (Phycoerythrin) images

  • Intracellular flow analysis of RANK in RAW cells using 0.5 µg of ab45092. Green histogram represents isotype control; red represents ab45092.

References for Anti-RANK antibody [9A725] (Phycoerythrin) (ab45092)

This product has been referenced in:
  • Kwan Tat S  et al. Modulation of OPG, RANK and RANKL by human chondrocytes and their implication during osteoarthritis. Rheumatology (Oxford) 48:1482-90 (2009). Read more (PubMed: 19762475) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I am pleased to provide the following information: We have two RANK antibodies tested flow cytometry, human and mouse (not IHC or rat): ab45092 RANK antibody [9A725] (Phycoerythrin) Mouse monoclonal Tested applicat...

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Application Flow Cytometry
Sample Mouse Cell (mouse macrophage RAW264)
Specification mouse macrophage RAW264
Preparation Cell harvesting/tissue preparation method: scrape off in cell dissociation solution (SIGMA) and washed by centrifugation
Sample buffer: wash buffer (3 % BSA, 1 % sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization Yes - 0.1 % Triton
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Dr. 拓哉 小川

Verified customer

Submitted Oct 28 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"