• Product nameAnti-RANKL antibody
    See all RANKL primary antibodies
  • Description
    Rabbit polyclonal to RANKL
  • Specificityab65024 detects endogenous levels of total RANKL protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human RANKL

  • Positive control
    • HuvEc cell extracts and HepG2 cells.



Our Abpromise guarantee covers the use of ab65024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21742639


  • FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localizationCytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD254 antibody
    • hRANKL2 antibody
    • ODF antibody
    • OPGL antibody
    • OPTB2 antibody
    • Osteoclast differentiation factor antibody
    • Osteoprotegerin ligand antibody
    • RANKL antibody
    • Receptor activator of nuclear factor kappa B ligand antibody
    • Receptor activator of nuclear factor kappa-B ligand antibody
    • sOdf antibody
    • TNF related activation induced cytokine antibody
    • TNF-related activation-induced cytokine antibody
    • TNF11_HUMAN antibody
    • TNFSF 11 antibody
    • Tnfsf11 antibody
    • TRANCE antibody
    • Tumor necrosis factor (ligand) superfamily member 11 antibody
    • Tumor necrosis factor ligand superfamily member 11 antibody
    • Tumor necrosis factor ligand superfamily member 11, soluble form antibody
    see all

Anti-RANKL antibody images

  • ab65024 at 1/500 - 1/1000 dilution staining RANKL in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-RANKL antibody (ab65024) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-RANKL antibody (ab65024)

This product has been referenced in:
  • Yeo L  et al. Expression of FcRL4 defines a pro-inflammatory, RANKL-producing B cell subset in rheumatoid arthritis. Ann Rheum Dis N/A:N/A (2014). Human . Read more (PubMed: 24431391) »
  • Laury AM  et al. Periostin and receptor activator of nuclear factor ?-B ligand expression in allergic fungal rhinosinusitis. Int Forum Allergy Rhinol 4:716-24 (2014). Read more (PubMed: 25060295) »

See all 5 Publications for this product

Product Wall

Thank you for your reply.

The amino acid range of this immunogen is aa 60-110 of human Tumor necrosis factor ligand super family member 11 with accession number O14788 (http://www.uniprot.org/uniprot/O14788). This antibody binds to the C-term...

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I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:


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Thank you for your reply.

I am sorry to hear this product is not satisfactory. In many cases changing the protocol conditions does improve the results especially if a product is working in 1 application but not another. Although a different ...

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Thank you for your enquiry regarding ab65024 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

As I understand the antibody worked fine on HepG2...

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