Anti-RANKL antibody [IK22/5] (Phycoerythrin) (ab93553)

Overview

  • Product name
    Anti-RANKL antibody [IK22/5] (Phycoerythrin)
    See all RANKL primary antibodies
  • Description
    Rat monoclonal [IK22/5] to RANKL (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    NSO derived recombinant mouse RANKL.

  • Positive control
    • 3-day ConA activated BALB/c splenocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab93553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at a concentration of 0.125 - 0.25 µg/test.
    A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
    • Tissue specificity
      Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
    • Involvement in disease
      Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
    • Sequence similarities
      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
    • Cellular localization
      Cytoplasm; Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD254 antibody
      • hRANKL2 antibody
      • ODF antibody
      • OPGL antibody
      • OPTB2 antibody
      • Osteoclast differentiation factor antibody
      • Osteoprotegerin ligand antibody
      • RANKL antibody
      • Receptor activator of nuclear factor kappa B ligand antibody
      • Receptor activator of nuclear factor kappa-B ligand antibody
      • sOdf antibody
      • TNF related activation induced cytokine antibody
      • TNF-related activation-induced cytokine antibody
      • TNF11_HUMAN antibody
      • TNFSF 11 antibody
      • Tnfsf11 antibody
      • TRANCE antibody
      • Tumor necrosis factor (ligand) superfamily member 11 antibody
      • Tumor necrosis factor ligand superfamily member 11 antibody
      • Tumor necrosis factor ligand superfamily member 11, soluble form antibody
      see all

    Images

    • ab93553 at 0.125µg dilution staining RANKL in 3-day unstimulated BALB/c splenocytes by Flow Cytometry (shown in blue).
    • ab93553 at 0.125µg dilution staining RANKL in 3-day ConA activated BALB/c splenocytes by Flow Cytometry (shown in blue).

    References

    ab93553 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    No
    Specification
    fibroblasts
    Blocking step
    Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    no fixation
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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