Overview

  • Product nameAnti-RANTES antibody
    See all RANTES primary antibodies
  • Description
    Rabbit polyclonal to RANTES
  • Tested applicationsSuitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hRANTES (human RANTES)

Properties

Applications

Our Abpromise guarantee covers the use of ab9679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Can be paired for ELISA with Mouse monoclonal [VL-1] to RANTES (ab9666).
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
IHC-P Use a concentration of 2 µg/ml.

Target

  • FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificityT-cell and macrophage specific.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

Anti-RANTES antibody images

  • ab9679 staining RANTES in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6) and tris buffer (pH 8). Samples were incubated with primary antibody (2μg/ml) for 1 hour at 25°C. An undiluted HRP-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab9679 staining RANTES in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.0 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an AEC chromogen.

References for Anti-RANTES antibody (ab9679)

This product has been referenced in:
  • Fan Y  et al. Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion. Mediators Inflamm 2014:397815 (2014). IHC-P ; Human . Read more (PubMed: 24808637) »
  • Galvão AM  et al. Immune-endocrine cross-talk in reproductive biology and pathology. Mediators Inflamm 2014:856465 (2014). IHC . Read more (PubMed: 24872600) »

See all 7 Publications for this product

Product Wall

Abreviews
Application ELISA
Sample Human Recombinant protein (recombinant human RANTES was used for calibration)
Specification recombinant human RANTES was used for calibration
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Sandwich (Capture)
Username

Ms. Tatiana Karakasheva

Verified customer

Submitted Nov 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample Human Tissue sections (esophagus)
Specification esophagus
Permeabilization No
Fixative Formaldehyde
Username

Ms. Tatiana Karakasheva

Verified customer

Submitted Oct 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both Cit (pH6) and Tris (pH8) work well
Sample Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Methacarn
Username

Abcam user community

Verified customer

Submitted Jul 31 2013

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

The exception is the rabbit mo...

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Thank you for contacting us.
I have been in contact with the lab, I can confirm that ab9679 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the anti...

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Thank you for your enquiry.

For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a c...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (H23 cell line)
Loading amount 15 µg
Specification H23 cell line
Treatment Proprietary compound (6uM for 72H)
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"