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Our Abpromise guarantee covers the use of ab9666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/300. PubMed: 19155524|
|ELISA||1/8000. Can be paired for ELISA with Rabbit polyclonal to RANTES (ab9679).|
|Neutralising||Use a concentration of 5 - 7 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.|
ab9666 staining RANTES in human esophageal squamous cell carcinoma tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blockedwith a blocking agent for 20 minutes at 20°C and no antigen retrieval was conducted. The sample was incubated with primary antibody (1/100 in PBS + 0.1%BSA + 0.2%TritonX-100) at 4°C for 17 hours. A biotin-conjugated horse anti-mouse polyclonal (1/200) was used as the secondary antibody.