• Product nameAnti-RANTES antibody [VL-1]
    See all RANTES primary antibodies
  • Description
    Mouse monoclonal [VL-1] to RANTES
  • Tested applicationsSuitable for: IHC-P, ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).


Associated products


Our Abpromise guarantee covers the use of ab9666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300. PubMed: 19155524
ELISA 1/8000. Can be paired for ELISA with Rabbit polyclonal to RANTES (ab9679).
Neutralising Use a concentration of 5 - 7 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.


  • FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificityT-cell and macrophage specific.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

Anti-RANTES antibody [VL-1] images

  • ab9666 staining RANTES in human esophageal squamous cell carcinoma tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blockedwith a blocking agent for 20 minutes at 20°C and no antigen retrieval was conducted. The sample was incubated with primary antibody (1/100 in PBS + 0.1%BSA + 0.2%TritonX-100) at 4°C for 17 hours. A biotin-conjugated horse anti-mouse polyclonal (1/200) was used as the secondary antibody.

References for Anti-RANTES antibody [VL-1] (ab9666)

This product has been referenced in:
  • Tan MC  et al. Disruption of CCR5-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer. J Immunol 182:1746-55 (2009). IHC-P ; Human . Read more (PubMed: 19155524) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (esophageal squamous cell carcinoma)
Antigen retrieval step None - Buffer/Enzyme Used: 10mM citrate, pH6.0
Specification esophageal squamous cell carcinoma
Blocking step Non-Abcam Blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde

Ms. Tatiana Karakasheva

Verified customer

Submitted Feb 22 2016

Application ELISA
Sample Human Cell (tissue culture conditioned media was harvested to)
Specification tissue culture conditioned media was harvested to
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Sandwich (Capture)

Ms. Tatiana Karakasheva

Verified customer

Submitted Nov 04 2015