Overview

  • Product nameAnti-RANTES antibody [VL1]
    See all RANTES primary antibodies
  • Description
    Mouse monoclonal [VL1] to RANTES
  • SpecificityRecognizes natural and recombinant human RANTES.
  • Tested applicationsSuitable for: IHC-Fr, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • General notes


    This antibody is useful for studies involving inhibition of chemotaxis and immunostaining of cryostat sections and cytospins.

Properties

Applications

Our Abpromise guarantee covers the use of ab52562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificityT-cell and macrophage specific.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

References for Anti-RANTES antibody [VL1] (ab52562)

This product has been referenced in:
  • de Souza A  et al. Resolution of CD8+ lymphomatoid papulosis after surgical excision of the type AB-thymoma. Am J Dermatopathol 31:475-9 (2009). Read more (PubMed: 19542925) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization Yes - Triton X-100 in PBS
Specification Skin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted May 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"