Anti-RAP1 antibody [78B356] (ab13889)

Overview

  • Product name
    Anti-RAP1 antibody [78B356]
    See all RAP1 primary antibodies
  • Description
    Mouse monoclonal [78B356] to RAP1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    KAEEDPEAADSGEPQNKRTPDLPE

    , corresponding to amino acids 212-235 of Human RAP1.

  • Positive control
    • 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 50 kDa.

    Is unsuitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
    • Tissue specificity
      Ubiquitous. Highly expressed.
    • Sequence similarities
      Belongs to the RAP1 family.
      Contains 1 BRCT domain.
      Contains 1 Myb-like domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dopamine receptor interacting protein 5 antibody
      • Dopamine receptor-interacting protein 5 antibody
      • DRIP 5 antibody
      • DRIP5 antibody
      • hRap1 antibody
      • MGC105533 antibody
      • RAP 1 antibody
      • RAP1 homolog antibody
      • RAP1, yeast, homolog if antibody
      • RAP1, yeast, homolog of antibody
      • Repressor/activator protein 1 homolog antibody
      • TE2IP_HUMAN antibody
      • Telomeric Repeat Binding Factor 2 Interacting Protein antibody
      • Telomeric repeat-binding factor 2-interacting protein 1 antibody
      • TERF2-interacting protein antibody
      • TERF2-interacting telomeric protein 1 antibody
      • TERF2IP antibody
      • TRF2 Interacting Telomeric Protein RAP1 antibody
      • TRF2 interacting telomeric RAP1 protein antibody
      • TRF2-interacting telomeric protein 1 antibody
      • TRF2-interacting telomeric protein antibody
      see all

    Images

    • Detection of hRap1 in 293 cells: 2 ug/ml of ab13889 were used; a protein band of approximately 50 kDa is detected.

    References

    ab13889 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Dec 15 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up