Overview

  • Product nameAnti-RAP1 antibody
    See all RAP1 primary antibodies
  • Description
    Rabbit polyclonal to RAP1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 100-150 of Human RAP1 (NP_061848.2).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab125528 is purified using an epitope specific to RAP1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionActs both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
  • Tissue specificityUbiquitous. Highly expressed.
  • Sequence similaritiesBelongs to the RAP1 family.
    Contains 1 BRCT domain.
    Contains 1 Myb-like domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dopamine receptor interacting protein 5 antibody
    • Dopamine receptor-interacting protein 5 antibody
    • DRIP 5 antibody
    • DRIP5 antibody
    • hRap1 antibody
    • MGC105533 antibody
    • RAP 1 antibody
    • RAP1 homolog antibody
    • RAP1, yeast, homolog if antibody
    • RAP1, yeast, homolog of antibody
    • Repressor/activator protein 1 homolog antibody
    • TE2IP_HUMAN antibody
    • Telomeric Repeat Binding Factor 2 Interacting Protein antibody
    • Telomeric repeat-binding factor 2-interacting protein 1 antibody
    • TERF2-interacting protein antibody
    • TERF2-interacting telomeric protein 1 antibody
    • TERF2IP antibody
    • TRF2 Interacting Telomeric Protein RAP1 antibody
    • TRF2 interacting telomeric RAP1 protein antibody
    • TRF2-interacting telomeric protein 1 antibody
    • TRF2-interacting telomeric protein antibody
    see all

Anti-RAP1 antibody images

  • All lanes : Anti-RAP1 antibody (ab125528) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 44 kDa


    Exposure time : 3 minutes
  • Detection of RAP1 by Western Blot of Immunprecipitate.
    ab125528 at 1µg/ml staining RAP1 in HeLa whole cell lysate immunoprecipitated using ab125528 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-RAP1 antibody (ab125528)

ab125528 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125528.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"