Anti-RAP1A antibody [EP878] (ab124963)
Key features and details
- Rabbit monoclonal [EP878] to RAP1A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-RAP1A antibody [EP878]
See all RAP1A primary antibodies -
Description
Rabbit monoclonal [EP878] to RAP1A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human brain, BXPC 3, C6, and NIH 3T3 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP878 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5906 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- SwissProt: P62834 Human
- SwissProt: P62835 Mouse
- SwissProt: P62836 Rat
- Unigene: 190334 Human
see all -
Alternative names
- C21KG antibody
- G 22K antibody
- G-22K antibody
see all
Images
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All lanes : Anti-RAP1A antibody [EP878] (ab124963) at 1/1000 dilution
Lane 1 : Human brain cell lysates
Lane 2 : BXPC 3 cell lysates
Lane 3 : C6 cell lysates
Lane 4 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124963 has not yet been referenced specifically in any publications.