Anti-RAP1A G12V antibody [EP878] (ab124963)

Overview

  • Product name
    Anti-RAP1A G12V antibody [EP878]
  • Description
    Rabbit monoclonal [EP878] to RAP1A G12V
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RAP1A G12V.

  • Positive control
    • Human brain, BXPC 3, C6, and NIH 3T3 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP878
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Ras family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI848598 antibody
      • C21KG antibody
      • G 22K antibody
      • G-22K antibody
      • GTP binding protein SMG P21A antibody
      • GTP-binding protein smg p21A antibody
      • KREV 1 antibody
      • KREV1 antibody
      • MGC102329 antibody
      • MGC93785 antibody
      • MGC94873 antibody
      • OTTHUMP00000013741 antibody
      • RAP1 antibody
      • RAP1A antibody
      • RAP1A, member of RAS oncogene family antibody
      • RAP1A_HUMAN antibody
      • RAS related protein 1a antibody
      • Ras related protein Krev 1 antibody
      • Ras related protein Rap 1A antibody
      • RAS related protein RAP1A antibody
      • Ras-related protein Krev-1 antibody
      • Ras-related protein Rap-1A antibody
      • SMGP21 antibody
      see all

    Images

    • All lanes : Anti-RAP1A G12V antibody [EP878] (ab124963) at 1/1000 dilution

      Lane 1 : Human brain cell lysates
      Lane 2 : BXPC 3 cell lysates
      Lane 3 : C6 cell lysates
      Lane 4 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa

    References

    ab124963 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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