Recombinant Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP879Y] to RAP1A + RAP1B
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAP1A + RAP1B antibody [EP879Y]
See all RAP1A + RAP1B primary antibodies -
Description
Rabbit monoclonal [EP879Y] to RAP1A + RAP1B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP879Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Relevance
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
Cellular localization
Cell Membrane; Attached to the membrane by a lipid anchor -
Database links
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
see all -
Alternative names
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
see all
Images
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Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaSecondary antibody - goat anti-rabbit HRP (ab6721)
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Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain
Predicted band size: 21 kDaSecondary antibody - goat anti-rabbit HRP (ab6721)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab75871 has been referenced in 3 publications.
- Cho EA et al. Phosphorylation of RIAM by src promotes integrin activation by unmasking the PH domain of RIAM. Structure 29:320-329.e4 (2021). PubMed: 33275877
- Xu B et al. GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose. Brain Res Bull 142:163-175 (2018). PubMed: 30031816
- Todorovic V et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). PubMed: 25208567