Overview

  • Product name
  • Description
    Rabbit polyclonal to RAP1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-156 of Human RAP1B (UniProt: P61224).

  • Positive control
    • HCT116 whole cell lysate; Human lung adenocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) .

Target

Images

  • Anti-RAP1B antibody (ab154756) at 1/500 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 20 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RAP1B with ab154756 at 1/100 dilution.

References

ab154756 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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