Overview

  • Product nameAnti-RAP1GAP antibody [Y135]
    See all RAP1GAP primary antibodies
  • Description
    Rabbit monoclonal [Y135] to RAP1GAP
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C terminus of human Rap1GAP.

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human stomach carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • FunctionGTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
  • Tissue specificitySignificant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
  • Sequence similaritiesContains 1 GoLoco domain.
    Contains 1 Rap-GAP domain.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0474 antibody
    • Rap1 GTPase activating protein 1 antibody
    • RAP1 GTPase activating protein antibody
    • Rap1 GTPase-activating protein 1 antibody
    • RAP1GA1 antibody
    • Rap1ga1 protein antibody
    • Rap1GAP antibody
    • Rap1GAP1 antibody
    • RAP1GAPII antibody
    • RAPGAP antibody
    • RPGP1_HUMAN antibody
    see all

Anti-RAP1GAP antibody [Y135] images

  • Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution + Jurkat cell lysate

    Predicted band size : 73 kDa
    Observed band size : 85-95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.

References for Anti-RAP1GAP antibody [Y135] (ab32119)

ab32119 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"