The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml. Note: the product requires protein A purification prior to use for ICC/IF.
WB: 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.