Anti-RAP2B antibody (ab101369)
Key features and details
- Rabbit polyclonal to RAP2B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP2B antibody -
Description
Rabbit polyclonal to RAP2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Zebrafish -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 159 of Human RAP2B (AAH12362)
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Positive control
- A431 whole cell lysate. H1299 and HepG2 cell lines. CL1-0 Xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended |
Target
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Function
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. -
Tissue specificity
Expressed in red blood cells (at protein level). -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Domain
The effector domain mediates the interaction with RUNDC3A. -
Post-translational
modificationsPalmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK. -
Cellular localization
Recycling endosome membrane. Associated with red blood cells-released vesicles. - Information by UniProt
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Database links
- Entrez Gene: 515181 Cow
- Entrez Gene: 5912 Human
- Omim: 179541 Human
- SwissProt: Q08DI5 Cow
- SwissProt: P61225 Human
- Unigene: 98643 Human
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Alternative names
- MGC20484 antibody
- RAP 2B antibody
- RAP2A antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab101369 has been referenced in 3 publications.
- Han Y et al. lncRNA FLVCR1-AS1 drives colorectal cancer progression via modulation of the miR-381/RAP2A axis. Mol Med Rep 23:N/A (2021). PubMed: 33313944
- Shi G & Zhang Z Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway. Oncol Lett 21:314 (2021). PubMed: 33692846
- Di JH et al. Rap2B promotes migration and invasion of human suprarenal epithelioma. Tumour Biol 35:9387-94 (2014). PubMed: 24951956