The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.
Use a concentration of 1 µg/ml.
Use a concentration of 10 µg/ml.
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionSmall GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
Tissue specificityExpressed in red blood cells (at protein level).
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
DomainThe effector domain mediates the interaction with RUNDC3A.
Post-translational modificationsPalmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.
Cellular localizationRecycling endosome membrane. Associated with red blood cells-released vesicles.
ab58109 at 10 ug/ml staining RAP2B in human Hella cells by Immunocytochemistry/ Immunofluorescence.
Flow Cytometry-Anti-RAP2B antibody(ab58109)
Overlay histogram showing K562 cells stained with ab58109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58109, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-RAP2B antibody (ab58109)
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