Overview

  • Product name
  • Description
    Goat polyclonal to RAP80
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Pig, Monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide:

    C-TRRQKEAKTKSDSG

    , corresponding to internal sequence amino acids 535-548 of Human RAP80.

  • Positive control
    • Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab134798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. Preliminary experiments gave an approx 60 kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7 kDa according to NP_057374.3. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
  • Tissue specificity
    Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
  • Sequence similarities
    Belongs to the RAP80 family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Domain
    The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
    The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
  • Post-translational
    modifications
    Sumoylated.
    Phosphorylated upon DNA damage by ATM or ATR.
  • Cellular localization
    Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1-A complex subunit RAP80 antibody
    • Nuclear zinc finger protein RAP80 antibody
    • OTTHUMP00000161441 antibody
    • OTTHUMP00000223372 antibody
    • OTTHUMP00000223374 antibody
    • RAP80 antibody
    • Receptor associated protein 80 antibody
    • Receptor-associated protein 80 antibody
    • Retinoid X receptor interacting protein 110 antibody
    • Retinoid x receptor interacting protein antibody
    • Retinoid X receptor-interacting protein 110 antibody
    • RIP110 antibody
    • Rxrip110 antibody
    • Ubiquitin interaction motif containing 1 antibody
    • Ubiquitin interaction motif containing protein 1 antibody
    • Ubiquitin interaction motif-containing protein 1 antibody
    • UIMC1 antibody
    • UIMC1_HUMAN antibody
    • X2HRIP110 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human testis tissue labelling RAP80 with ab134798 at 3.75µg/ml.

References

ab134798 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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