• Product nameAnti-RAP80 antibody
    See all RAP80 primary antibodies
  • Description
    Mouse polyclonal to RAP80
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-385 of Human RAP80



Our Abpromise guarantee covers the use of ab67606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 80 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionUbiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
    • Tissue specificityExpressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
    • Sequence similaritiesBelongs to the RAP80 family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • DomainThe UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
      The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
    • Post-translational
      Phosphorylated upon DNA damage by ATM or ATR.
    • Cellular localizationNucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRCA1-A complex subunit RAP80 antibody
      • Nuclear zinc finger protein RAP80 antibody
      • OTTHUMP00000161441 antibody
      • OTTHUMP00000223372 antibody
      • OTTHUMP00000223374 antibody
      • RAP80 antibody
      • Receptor associated protein 80 antibody
      • Receptor-associated protein 80 antibody
      • Retinoid X receptor interacting protein 110 antibody
      • Retinoid x receptor interacting protein antibody
      • Retinoid X receptor-interacting protein 110 antibody
      • RIP110 antibody
      • Rxrip110 antibody
      • Ubiquitin interaction motif containing 1 antibody
      • Ubiquitin interaction motif containing protein 1 antibody
      • Ubiquitin interaction motif-containing protein 1 antibody
      • UIMC1 antibody
      • UIMC1_HUMAN antibody
      • X2HRIP110 antibody
      see all

    Anti-RAP80 antibody images

    • All lanes : Anti-RAP80 antibody (ab67606) at 1/500 dilution

      Lane 1 : RAP80 transfected 293T cell lysate with the recombinant fragment used as the immunogen
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

      Predicted band size : 80 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)

    References for Anti-RAP80 antibody (ab67606)

    ab67606 has not yet been referenced specifically in any publications.

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