• Product name
  • Description
    Rabbit polyclonal to RAP80
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).

  • Positive control
    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab70822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
    • Tissue specificity
      Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
    • Sequence similarities
      Belongs to the RAP80 family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • Domain
      The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
      The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
    • Post-translational
      Phosphorylated upon DNA damage by ATM or ATR.
    • Cellular localization
      Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRCA1-A complex subunit RAP80 antibody
      • Nuclear zinc finger protein RAP80 antibody
      • OTTHUMP00000161441 antibody
      • OTTHUMP00000223372 antibody
      • OTTHUMP00000223374 antibody
      • RAP80 antibody
      • Receptor associated protein 80 antibody
      • Receptor-associated protein 80 antibody
      • Retinoid X receptor interacting protein 110 antibody
      • Retinoid x receptor interacting protein antibody
      • Retinoid X receptor-interacting protein 110 antibody
      • RIP110 antibody
      • Rxrip110 antibody
      • Ubiquitin interaction motif containing 1 antibody
      • Ubiquitin interaction motif containing protein 1 antibody
      • Ubiquitin interaction motif-containing protein 1 antibody
      • UIMC1 antibody
      • UIMC1_HUMAN antibody
      • X2HRIP110 antibody
      see all

    Anti-RAP80 antibody images

    • All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate at 15 µg
      Lane 2 : HeLa whole cell lysate at 5 µg
      Lane 3 : 293T whole cell lysate at 50 µg

      Predicted band size : 80 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 300 kDa,65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    References for Anti-RAP80 antibody (ab70822)

    This product has been referenced in:
    • Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). Read more (PubMed: 25939603) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (MCF7 cells)
    MCF7 cells
    Yes - Acetone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jul 21 2010


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