Overview

  • Product nameAnti-RAP80 antibody [EPR5315]
    See all RAP80 primary antibodies
  • Description
    Rabbit monoclonal [EPR5315] to RAP80
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human RAP80.

  • Positive control
    • HeLa, Human ovary cancer, 293T and HT-1080 cell lysates; Human testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab124763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionUbiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
    • Tissue specificityExpressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
    • Sequence similaritiesBelongs to the RAP80 family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • DomainThe UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
      The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
    • Post-translational
      modifications
      Sumoylated.
      Phosphorylated upon DNA damage by ATM or ATR.
    • Cellular localizationNucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRCA1-A complex subunit RAP80 antibody
      • Nuclear zinc finger protein RAP80 antibody
      • OTTHUMP00000161441 antibody
      • OTTHUMP00000223372 antibody
      • OTTHUMP00000223374 antibody
      • RAP80 antibody
      • Receptor associated protein 80 antibody
      • Receptor-associated protein 80 antibody
      • Retinoid X receptor interacting protein 110 antibody
      • Retinoid x receptor interacting protein antibody
      • Retinoid X receptor-interacting protein 110 antibody
      • RIP110 antibody
      • Rxrip110 antibody
      • Ubiquitin interaction motif containing 1 antibody
      • Ubiquitin interaction motif containing protein 1 antibody
      • Ubiquitin interaction motif-containing protein 1 antibody
      • UIMC1 antibody
      • UIMC1_HUMAN antibody
      • X2HRIP110 antibody
      see all

    Anti-RAP80 antibody [EPR5315] images

    • All lanes : Anti-RAP80 antibody [EPR5315] (ab124763) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 80 kDa
    • ab124763, at 1/250, staining RAP80 in paraffin-embedded Human testis tissue by Immunohistochemistry.

    References for Anti-RAP80 antibody [EPR5315] (ab124763)

    This product has been referenced in:
    • Hu B  et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res 42:5582-93 (2014). Human . Read more (PubMed: 24711370) »
    • Kakarougkas A  et al. Co-operation of BRCA1 and POH1 relieves the barriers posed by 53BP1 and RAP80 to resection. Nucleic Acids Res N/A:N/A (2013). ICC/IF . Read more (PubMed: 24013561) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (EC109)
    Gel Running Conditions Non-reduced Denaturing (10% SDS)
    Loading amount 80 µg
    Specification EC109
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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    Submitted Dec 31 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"