Overview

  • Product name
  • Description
    Rabbit polyclonal to RAPH1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 3-75 (QLSDEEIDHG AEEDSDKEDQ DLDKMFGAWL GELDKLTQSL DSDKPMEPVK RSPLRQETNM ANFSYRFSIY NLN) of Human RAPH1.

  • Positive control
    • Human Skin tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.

Target

  • Function
    Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
  • Tissue specificity
    Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.
  • Sequence similarities
    Belongs to the MRL family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430025M21Rik antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein antibody
    • C730009O10Rik antibody
    • Lamellipodin antibody
    • PREL-2 antibody
    • Proline-rich EVH1 ligand 2 antibody
    • Protein RMO1 antibody
    • RAPH1 antibody
    • RAPH1_HUMAN antibody
    • Ras-associated and pleckstrin homology domains-containing protein 1 antibody
    see all

Images

  • ab121512 at 1/15 dilution staining RAPH1 in Paraffin-embedded Human Skin tissue by Immunohistochemistry.

References

ab121512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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