• Product nameAnti-RAPH1 antibody
    See all RAPH1 primary antibodies
  • Description
    Rabbit polyclonal to RAPH1
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to C terminal amino acids 1105-1248 of Human RAPH1.

  • Positive control
    • Human duodenum tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab121619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6


  • FunctionMediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
  • Tissue specificityIsoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.
  • Sequence similaritiesBelongs to the MRL family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
  • Cellular localizationCell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430025M21Rik antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein antibody
    • C730009O10Rik antibody
    • Lamellipodin antibody
    • PREL-2 antibody
    • Proline-rich EVH1 ligand 2 antibody
    • Protein RMO1 antibody
    • RAPH1 antibody
    • RAPH1_HUMAN antibody
    • Ras-associated and pleckstrin homology domains-containing protein 1 antibody
    see all

Anti-RAPH1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane and cytoplasm. Recommended concentration of ab121619 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121619 at 1/75 staining RALPH1 in Paraffin Embedded Human duodenum tissue. Note strong cytoplasmic positivity in glandular cells.

References for Anti-RAPH1 antibody (ab121619)

ab121619 has not yet been referenced specifically in any publications.

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