Overview

  • Product nameAnti-Raptor antibody
    See all Raptor primary antibodies
  • Description
    Rabbit polyclonal to Raptor
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Pig, Opossum, Chinese Hamster, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of Raptor (regulatory associated protein of mTOR) encoded within exon 26 according to the human gene (LocusLink ID 57521).

Properties

Applications

Our Abpromise guarantee covers the use of ab5454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23982838

Target

  • FunctionInvolved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
  • Tissue specificityHighly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
  • Sequence similaritiesBelongs to the WD repeat RAPTOR family.
    Contains 7 WD repeats.
  • Cellular localizationCytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1303 antibody
    • KOG1 antibody
    • Mip1 antibody
    • P150 target of rapamycin (TOR) scaffold protein antibody
    • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
    • P150 target of rapamycin (TOR)-scaffold protein antibody
    • Raptor antibody
    • Regulatory associated protein of mTOR antibody
    • Regulatory associated protein of MTOR complex 1 antibody
    • Regulatory-associated protein of mTOR antibody
    • RPTOR antibody
    • RPTOR_HUMAN antibody
    see all

Anti-Raptor antibody images



  • Predicted band size : 150 kDa


    Detection of Human, Mouse and Rat Raptor by Western Blot. Samples: Whole cell lysate (20 mcg) from rat L6, mouse C2C12 and human HEK293 cells (lanes 1, 2 and 3, respectively).

References for Anti-Raptor antibody (ab5454)

This product has been referenced in:
  • Kim SG  et al. Bilirubin activates transcription of HIF-1a in human proximal tubular cells cultured in the physiologic oxygen content. J Korean Med Sci 29 Suppl 2:S146-54 (2014). WB ; Human . Read more (PubMed: 25317019) »
  • Chung SH  et al. Laser capture microdissection-directed profiling of glycolytic and mTOR pathways in areas of selectively ablated Müller cells in the murine retina. Invest Ophthalmol Vis Sci 54:6578-85 (2013). ICC/IF ; Mouse . Read more (PubMed: 23982838) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Xenopus laevis Cell lysate - other (stage 35/36 head lysate (brain+eyes))
Loading amount 20 µg
Specification stage 35/36 head lysate (brain+eyes)
Gel Running Conditions Non-reduced Denaturing (5% polycrylamide gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Bladder cancer cells)
Loading amount 40 µg
Specification Bladder cancer cells
Gel Running Conditions Non-reduced Denaturing (8 %)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. santosh chauhan

Verified customer

Submitted Aug 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 myoblast)
Loading amount 10 µg
Specification C2C12 myoblast
Treatment +/- siRNA against Raptor
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Hilary Pollard

Verified customer

Submitted Jun 02 2011

Thank you for your enquiry. Ab5454 was characterized in Western blotting using whole cell lysate (20 mcg) from rat L6, mouse C2C12 and human HEK293 cells, and ab5454 was used at 0.2 mcg/ml. The specific Western blotting protocol that was used is not av...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"