Anti-Raptor antibody (ab62454)
Key features and details
- Rabbit polyclonal to Raptor
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Raptor antibody
See all Raptor primary antibodies -
Description
Rabbit polyclonal to Raptor -
Host species
Rabbit -
Specificity
This antibody reacts with Raptor. Raptor has multiple isoforms that may also be recognized by this antibody. -
Tested applications
Suitable for: WB, ICC/IF, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
A 15 amino acid synthetic peptide from the central region of human Raptor
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Positive control
- L1210 cell lysate and L1210 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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ICC |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ICC
Use a concentration of 1 µg/ml. |
Target
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Function
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
Tissue specificity
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
Sequence similarities
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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Database links
- Entrez Gene: 74370 Mouse
- SwissProt: Q8K4Q0 Mouse
- Unigene: 209933 Mouse
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Alternative names
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
Images
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Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/ml
Lane 2 : Anti-Raptor antibody (ab62454) at 4 µg/ml
All lanes : L1210 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa -
Immunocytochemical staining of L1210 cells using Raptor antibody at 1µg/ml.
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Immunofluorescence of Raptor in L1210 cells using ab62454 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab62454 has not yet been referenced specifically in any publications.