Overview

  • Product nameAnti-Raptor antibody
    See all Raptor primary antibodies
  • Description
    Rabbit polyclonal to Raptor
  • SpecificityThis antibody reacts with Raptor. Raptor has multiple isoforms that may also be recognized by this antibody.
  • Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid synthetic peptide from the central region of human Raptor

  • Positive control
    • L1210 cell lysate and L1210 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
  • Tissue specificityHighly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
  • Sequence similaritiesBelongs to the WD repeat RAPTOR family.
    Contains 7 WD repeats.
  • Cellular localizationCytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1303 antibody
    • KOG1 antibody
    • Mip1 antibody
    • P150 target of rapamycin (TOR) scaffold protein antibody
    • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
    • P150 target of rapamycin (TOR)-scaffold protein antibody
    • Raptor antibody
    • Regulatory associated protein of mTOR antibody
    • Regulatory associated protein of MTOR complex 1 antibody
    • Regulatory-associated protein of mTOR antibody
    • RPTOR antibody
    • RPTOR_HUMAN antibody
    see all

Anti-Raptor antibody images

  • Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/ml
    Lane 2 : Anti-Raptor antibody (ab62454) at 4 µg/ml

    Lane 1 : L1210 cell lysate
    Lane 2 : L1210 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 149 kDa
    Observed band size : 149 kDa
  • Immunocytochemical staining of L1210 cells using Raptor antibody at 1µg/ml.
  • Immunofluorescence of Raptor in L1210 cells using ab62454 at 10 ug/ml.

References for Anti-Raptor antibody (ab62454)

ab62454 has not yet been referenced specifically in any publications.

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