Overview

  • Product nameAnti-Raptor antibody [EP539Y]
    See all Raptor primary antibodies
  • Description
    Rabbit monoclonal [EP539Y] to Raptor
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Raptor (C terminal).

  • Positive control
    • Normal human colon and Human cardiac muscle; mouse and rat brain tissue lysate HEK-293, HeLa, MCF-7 and A431 cell lysate;
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab40768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 149 kDa).

For unpurified use at 1/500. 

IHC-P 1/100.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionInvolved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
    • Tissue specificityHighly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
    • Sequence similaritiesBelongs to the WD repeat RAPTOR family.
      Contains 7 WD repeats.
    • Cellular localizationCytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1303 antibody
      • KOG1 antibody
      • Mip1 antibody
      • P150 target of rapamycin (TOR) scaffold protein antibody
      • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
      • P150 target of rapamycin (TOR)-scaffold protein antibody
      • Raptor antibody
      • Regulatory associated protein of mTOR antibody
      • Regulatory associated protein of MTOR complex 1 antibody
      • Regulatory-associated protein of mTOR antibody
      • RPTOR antibody
      • RPTOR_HUMAN antibody
      see all

    Anti-Raptor antibody [EP539Y] images

    • All lanes : Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution

      Lane 1 : HEK-293 (human embryonic kidney) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 3 : MCF-7 (human breast carcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM /TBST
    • Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution + Rat brain tissue lysate at 20 mg/ml

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM /TBST
    • Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.
    • Anti-Raptor antibody [EP539Y] (ab40768) at 1/1000 dilution + Mouse brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM /TBST
    • All lanes : Anti-Raptor antibody [EP539Y] (ab40768) at 1/500 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 149 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 21 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Raptor antibody [EP539Y] (ab40768)

    This product has been referenced in:
    • Dey-Guha I  et al. A mechanism for asymmetric cell division resulting in proliferative asynchronicity. Mol Cancer Res 13:223-30 (2015). WB ; Human . Read more (PubMed: 25582703) »
    • Rodgers JT  et al. mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert). Nature 509:393-6 (2014). Mouse . Read more (PubMed: 24870234) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Muscle)
    Loading amount 50 µg
    Specification Muscle
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2012

    Thank you very much for taking the time to complete and send the questionnaire.

    The details you have kindly provided will provide us with vital information for our monitoring of product quality.

    Unfortunately none of these antibodie...

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    In or...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Liver)
    Loading amount 40 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 09 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Mouse Tissue lysate - whole (Liver)
    Total protein in input 3000 µg
    Specification Liver
    Immuno-precipitation step Protein A
    Username

    Abcam user community

    Verified customer

    Submitted Mar 09 2012

    Thank you for contacting Abcam regarding ab40768. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the...

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    Thank you for contacting us. The secondary antibodies can be used within range from 1/1000 to 1/50,000 dilution. The dilution range depends on the concentration of secondary antibody. We recommend starting with 1/1000, 1/2000, 1/5000 and 1/10,000 f...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"