Overview

  • Product nameAnti-RARRES3 antibody
    See all RARRES3 primary antibodies
  • Description
    Mouse monoclonal to RARRES3
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human RARRES3 with tag (aa 1-165; AAH09678).

  • Positive control
    • RARRES3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.
IHC-P 1/20. PubMed: 24886842
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionExhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. N- and O-acylation activity is hardly detectable.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the H-rev107 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • HRASLS4 antibody
    • MGC8906 antibody
    • RAR responsive protein TIG3 antibody
    • RAR-responsive protein TIG3 antibody
    • RARRES3 antibody
    • Retinic acid inducible gene 1 antibody
    • Retinoic acid receptor responder (tazarotene induced) 3 antibody
    • Retinoic acid receptor responder protein 3 antibody
    • Retinoid inducible gene 1 protein antibody
    • Retinoid-inducible gene 1 protein antibody
    • RIG 1 antibody
    • rig1 antibody
    • Tazarotene induced gene 3 protein antibody
    • Tazarotene-induced gene 3 protein antibody
    • TIG 3 antibody
    • TIG3 antibody
    • TIG3_HUMAN antibody
    see all

Anti-RARRES3 antibody images

  • All lanes : Anti-RARRES3 antibody (ab77010) at 1/500 dilution

    Lane 1 : RARRES3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Overlay histogram showing MOLT4 cells stained with ab77010 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77010, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-RARRES3 antibody (ab77010)

This product has been referenced in:
  • Tengroth L  et al. Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5 stimulation in nasal epithelial cells. PLoS One 9:e98239 (2014). IHC-P, Flow Cyt ; Human . Read more (PubMed: 24886842) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"