The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Use at an assay dependent concentration.
1/20. PubMed: 24886842
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionExhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. N- and O-acylation activity is hardly detectable.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the H-rev107 family.
Retinoic acid receptor responder protein 3 antibody
Retinoid inducible gene 1 protein antibody
Retinoid-inducible gene 1 protein antibody
RIG 1 antibody
Tazarotene induced gene 3 protein antibody
Tazarotene-induced gene 3 protein antibody
TIG 3 antibody
Anti-RARRES3 antibody images
Western blot - RARRES3 antibody (ab77010)
All lanes : Anti-RARRES3 antibody (ab77010) at 1/500 dilution
Lane 1 : RARRES3 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 18 kDa Observed band size : 18 kDa
Flow Cytometry - Anti-RARRES3 antibody (ab77010)
Overlay histogram showing MOLT4 cells stained with ab77010 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77010, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-RARRES3 antibody (ab77010)
This product has been referenced in:
Tengroth L et al. Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5 stimulation in nasal epithelial cells. PLoS One9:e98239 (2014).
IHC-P, Flow Cyt
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