The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 66 kDa.
Involvement in disease
Defects in RARS2 are the cause of pontocerebellar hypoplasia type 6 (PCH6) [MIM:611523]; also known as fatal infantile encephalopathy with mitochondrial respiratory chain defects. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue, labeling RARS2 with ab175978 at 1/10 dilution. ab175978 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.