Overview

  • Product nameAnti-RASA1 antibody [B4F8]
    See all RASA1 primary antibodies
  • Description
    Mouse monoclonal [B4F8] to RASA1
  • SpecificityThis antibody is specific for RASA1 (an alternate name of GAP).
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Recombinant full length protein corresponding to Human RASA1.

  • EpitopeEpitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.
  • Positive control
    • RS-2 cell lysate. Mouse fibroblast cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesGTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.
  • ClonalityMonoclonal
  • Clone numberB4F8
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.

Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.

IP Use at an assay dependent concentration.

1-5µg.

This antibody immunoprecipitates GAP complexed with GAP related p190.

WB Use a concentration of 10 µg/ml.

By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate.

Target

  • FunctionInhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
  • Tissue specificityIn placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
  • Involvement in diseaseNote=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
    Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
    Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CM AVM antibody
    • CMAVM antibody
    • DKFZp434N071 antibody
    • GAP antibody
    • GTPase activating protein antibody
    • GTPase-activating protein antibody
    • OTTHUMP00000222390 antibody
    • OTTHUMP00000222391 antibody
    • OTTHUMP00000222392 antibody
    • OTTHUMP00000222393 antibody
    • p120GAP antibody
    • p120RASGAP antibody
    • PKWS antibody
    • Ras GTPase-activating protein 1 antibody
    • RAS p21 protein activator (GTPase activating protein) 1 antibody
    • Ras p21 protein activator antibody
    • RASA antibody
    • RASA1 antibody
    • RASA1_HUMAN antibody
    • RasGAP antibody
    • Triphosphatase activating protein antibody
    see all

Anti-RASA1 antibody [B4F8] images

  • ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse brain cell lysate

    Lysates/proteins at 25 µg per lane.


    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References for Anti-RASA1 antibody [B4F8] (ab2922)

This product has been referenced in:
  • Fiedler J  et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . Read more (PubMed: 21788589) »
  • Settleman J  et al. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell 69:539-49 (1992). Read more (PubMed: 1581965) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"