Recombinant
RabMAb

Anti-RASA1 antibody [EP536Y] (ab40677)

Overview

  • Product name
    Anti-RASA1 antibody [EP536Y]
    See all RASA1 primary antibodies
  • Description
    Rabbit monoclonal [EP536Y] to RASA1
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GAP aa 250-350 (internal sequence). From near the SH3 domain.
    Database link: P20936

  • Positive control
    • WB: Rat brain tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).
IHC-P 1/50.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
    • Tissue specificity
      In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
    • Involvement in disease
      Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
      Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
      Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
    • Sequence similarities
      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    Images

    • Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain lysate at 10 µg

      Predicted band size : 96 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cleavage fragment).
    • Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.

    References

    This product has been referenced in:
    • Teng HW  et al. Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma. Sci Rep 6:35308 (2016). Read more (PubMed: 27752061) »
    • Ying W  et al. MicroRNA-223 is a crucial mediator of PPAR?-regulated alternative macrophage activation. J Clin Invest 125:4149-59 (2015). Read more (PubMed: 26436647) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry.

    I can confirm the ICC testing protocol for which I have copied below. I hope this will be helpful. Please note, this would be a guideline only and may require some individual optimization.

    With regards ...

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