Recombinant
RabMAb

Anti-RASA4 antibody [EPR10515] (ab157190)

Overview

  • Product name
    Anti-RASA4 antibody [EPR10515]
    See all RASA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR10515] to RASA4
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RASA4 (UniProt: O43374).

  • Positive control
    • Human skeletal muscle, HepG2, 293T and HeLa cell lysates; Human brain and skeletal muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab157190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Relevance
      RASA4 is a Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium.
    • Cellular localization
      Cytoplasm; cytosol. Cell membrane; peripheral membrane protein. Note=Localized to the cytosol as a result of its lack of phosphoinositide binding activity. Upon agonist-stimulated calcium mobilization, utilizes the C2A and C2B domains to associate with the plasma membrane.
    • Database links
    • Alternative names
      • Ca2+ promoted Ras inactivator antibody
      • Calcium promoted Ras inactivator antibody
      • CAPRI antibody
      • GAPL antibody
      • GTPase activating protein like antibody
      • KIAA0538 antibody
      • MGC131890 antibody
      • Ras GTPase activating protein 4 antibody
      • RAS p21 protein activator 4 antibody
      • RasGAP activating like protein 2 antibody
      see all

    Images

    • ab157190 showing +ve staining in Mouse kidney.
    • ab157190 showing +ve staining in Human thyroid gland carcinoma.
    • ab157190 showing +ve staining in Human ovarian carcinoma.
    • ab157190 showing +ve staining in Human heart.
    • All lanes : Anti-RASA4 antibody [EPR10515] (ab157190) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : HepG2 lysate
      Lane 3 : 293T lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 90 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RASA4 with ab157190 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling RASA4 with ab157190 at 1/50 dilution.

    References

    ab157190 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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