Overview

  • Product name
  • Description
    Rabbit polyclonal to RASAL2
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 860-969 of Human RASAL2

  • Positive control
    • Human stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 129 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Images

  • Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling RASAL2 with ab121578 shows positive staining in the plasma membrane and focal adhesion sites.
  • All lanes : Anti-RASAL2 antibody (ab121578) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver
    Lane 5 : Human tonsil


    Predicted band size : 129 kDa
  • ab121578, at a 1/300 dilution, staining RASAL2 in paraffin-embedded Human stomach tissue by immunohistochemistry.

References

This product has been referenced in:
  • Huang Y  et al. RASAL2 down-regulation in ovarian cancer promotes epithelial-mesenchymal transition and metastasis. Oncotarget 5:6734-45 (2014). IHC ; Human . Read more (PubMed: 25216515) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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