Overview

  • Product nameAnti-RASD2 antibody
    See all RASD2 primary antibodies
  • Description
    Rabbit polyclonal to RASD2
  • Specificityab67277 may cross-react with the closely related protein RASD1.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the amino terminus of human RASD2.

  • Positive control
    • Rat colon tissue lysate. Human colon tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab67277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionGTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination.
  • Tissue specificityPancreatic endocrine cells (islets of Langerhans).
  • Sequence similaritiesBelongs to the small GTPase superfamily. RasD family.
  • Post-translational
    modifications
    Farnesylated. Farnesylation is required for membrane targeting.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein Rhes antibody
    • GTP-binding protein Rhes antibody
    • MGC:4834 antibody
    • OTTHUMP00000197925 antibody
    • Ras homolog enriched in striatum antibody
    • RASD 2 antibody
    • RASD family member 2 antibody
    • RASD2 antibody
    • Rhes antibody
    • RHES_HUMAN antibody
    • TEM 2 antibody
    • TEM2 antibody
    • Tumor endothelial marker 2 antibody
    see all

Anti-RASD2 antibody images

  • Lane 1 : Anti-RASD2 antibody (ab67277) at 1 µg/ml
    Lane 2 : Anti-RASD2 antibody (ab67277) at 2 µg/ml

    Lane 1 : Rat colon tissue lysate
    Lane 2 : Rat colon tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
  •  ab67277 at 2.5 μg/ml staining of RASD2 in human colon tissue using IHC-P.
  • Immunofluorescence of TEM2 in Human Colon cells using ab67277 at 20 ug/ml.

References for Anti-RASD2 antibody (ab67277)

ab67277 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"