Overview

  • Product nameAnti-RASD2 antibody
  • Description
    Rabbit polyclonal to RASD2
  • Specificityab67277 may cross-react with the closely related protein RASD1.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the amino terminus of human RASD2.

  • Positive control
    • Rat colon tissue lysate. Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionGTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination.
  • Tissue specificityPancreatic endocrine cells (islets of Langerhans).
  • Sequence similaritiesBelongs to the small GTPase superfamily. RasD family.
  • Post-translational
    modifications
    Farnesylated. Farnesylation is required for membrane targeting.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein Rhes antibody
    • GTP-binding protein Rhes antibody
    • MGC:4834 antibody
    • OTTHUMP00000197925 antibody
    • Ras homolog enriched in striatum antibody
    • RASD 2 antibody
    • RASD family member 2 antibody
    • RASD2 antibody
    • Rhes antibody
    • RHES_HUMAN antibody
    • TEM 2 antibody
    • TEM2 antibody
    • Tumor endothelial marker 2 antibody
    see all

Anti-RASD2 antibody images

  • Lane 1 : Anti-RASD2 antibody (ab67277) at 1 µg/ml
    Lane 2 : Anti-RASD2 antibody (ab67277) at 2 µg/ml

    Lane 1 : Rat colon tissue lysate
    Lane 2 : Rat colon tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
  •  ab67277 at 2.5 μg/ml staining of RASD2 in human colon tissue using IHC-P.
  • Immunofluorescence of TEM2 in Human Colon cells using ab67277 at 20 ug/ml.

References for Anti-RASD2 antibody (ab67277)

ab67277 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"