Overview

  • Product nameAnti-RASGRP 4 antibody
    See all RASGRP 4 primary antibodies
  • Description
    Mouse monoclonal to RASGRP 4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: CGLCCHKHCR DQVKVECKKR PGAKGDAGPP GAPVPSTPAP HASCGSEENH SYTLSLEPET GCQLRHAWTQ TESPHPSWET DTVPCPVMDP PSTASSKLDS , corresponding to amino acids 574-674 of Human RASGRP 4

Properties

Applications

Our Abpromise guarantee covers the use of ab57978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
    • Tissue specificityExpressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
    • Sequence similaritiesBelongs to the RASGRP family.
      Contains 1 EF-hand domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • Developmental stageExpressed early during development. Detected in all fetal tissues tested.
    • DomainThe phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
    • Cellular localizationCytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
    • Information by UniProt
    • Database links
    • Alternative names
      • GRP4_HUMAN antibody
      • Guanyl nucleotide releasing protein 4 antibody
      • RAS guanyl releasing protein 4 antibody
      • RAS guanyl-releasing protein 4 antibody
      • RASGRP4 antibody
      see all

    Anti-RASGRP 4 antibody images

    • RASGRP 4 antibody (ab57978) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab57978 RASGRP 4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-RASGRP 4 antibody (ab57978)

    ab57978 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"