Overview

  • Product name
  • Description
    Rabbit polyclonal to RASGRP 4
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 108 - 170 (KATGDTQELR RLQICHLVRY WLMRHPEVMH QDPQLEEVIG RFWATVAREG NSAQRRLGDS SDL) of Human RASGRP 4 (NP_733749).

  • Positive control
    • WB: A431, H1299, HeLa and Raji cell lysates IHC-P: SW480 (Human colon adenocarcinoma) tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.

Target

  • Function
    Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
  • Tissue specificity
    Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
  • Sequence similarities
    Belongs to the RASGRP family.
    Contains 1 EF-hand domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • Developmental stage
    Expressed early during development. Detected in all fetal tissues tested.
  • Domain
    The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
  • Cellular localization
    Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
  • Information by UniProt
  • Database links
  • Alternative names
    • GRP4_HUMAN antibody
    • Guanyl nucleotide releasing protein 4 antibody
    • RAS guanyl releasing protein 4 antibody
    • RAS guanyl-releasing protein 4 antibody
    • RASGRP4 antibody
    see all

Anti-RASGRP 4 antibody images

  • ICC/IF image of ab96293 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96293 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RASGRP 4 antibody (ab96293) at 1/1000 dilution + Raji whole cell lysates at 30 µg

    Predicted band size : 75 kDa
    7.5% SDS PAGE
  • ab96293, at a 1/100 dilution, staining RASGRP 4 in paraffin embedded SW480 (Human colon adenocarcinoma) tissue by Immunohistochemical analysis.

References for Anti-RASGRP 4 antibody (ab96293)

ab96293 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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