This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 90 kDa. PubMed: 25118589
IHC-P Use at an assay dependent concentration. Use at an assay dependent dilution

Target

  • Relevance
    Function: Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP (PubMed:15899849, PubMed:23908768). Activates the Erk/MAP kinase cascade (PubMed:15899849). Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras (PubMed:10807788, PubMed:12839994). Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed:19933860). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (By similarity). May also function in differentiation of other cell types (PubMed:12845332). Tissue specificity: Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level). Expressed in NK cells (at protein level) (PubMed:19933860). Disease: Systemic lupus erythematosus Similarity: Belongs to the RASGRP family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain. Developmental stage: Expressed in fetal brain and kidney. Domain: The phorbol-ester/DAG-type zinc finger is the principal mediator of the targeting to membranes and is required for functional activation through DAG-binding. Two EF-hand domains are present. However, only EF-hand 1 (and not EF-hand 2) binds calcium.
  • Cellular localization
    Cell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. Found in membrane fraction. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor.
  • Database links
  • Alternative names
    • Calcium and DAG-regulated guanine nucleotide exchange factor II antibody
    • CALDAG-GEFI antibody
    • CalDAG-GEFII antibody
    • GRP1_HUMAN antibody
    • Guanine nucleotide exchange factor, calcium and DAG regulated, Rap1A antibody
    • hRasGRP1 antibody
    • Ras activator RasGRP antibody
    • RAS guanyl nucleotide releasing protein 1 antibody
    • RAS guanyl releasing protein 1 (calcium and DAG-regulated) antibody
    • RAS guanyl releasing protein 1 antibody
    • RAS guanyl-releasing protein 1 antibody
    • Ras guanyl-releasing protein antibody
    • RASGRP antibody
    • rasgrp1 antibody
    • V antibody
    see all

References for Anti-Rasgrp1 antibody (ab37927)

This product has been referenced in:
  • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB ; Mouse . Read more (PubMed: 25118589) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum and Pons)
Specification
Cerebellum and Pons
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 05 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 15 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms Brain Ms Spleen Ms Stomach)
Loading amount
20 µg
Specification
Ms Brain Ms Spleen Ms Stomach
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jan 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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