Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
Use at an assay dependent concentration. Predicted molecular weight: 90 kDa. PubMed: 25118589
Use at an assay dependent concentration. Use at an assay dependent dilution
RelevanceFunction: Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP (PubMed:15899849, PubMed:23908768). Activates the Erk/MAP kinase cascade (PubMed:15899849). Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras (PubMed:10807788, PubMed:12839994). Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed:19933860). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (By similarity). May also function in differentiation of other cell types (PubMed:12845332).
Tissue specificity: Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level). Expressed in NK cells (at protein level) (PubMed:19933860).
Disease: Systemic lupus erythematosus
Similarity: Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Developmental stage: Expressed in fetal brain and kidney.
Domain: The phorbol-ester/DAG-type zinc finger is the principal mediator of the targeting to membranes and is required for functional activation through DAG-binding.
Two EF-hand domains are present. However, only EF-hand 1 (and not EF-hand 2) binds calcium.
Cellular localizationCell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. Found in membrane fraction. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor.