The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
Developmental stageExpressed in fetal brain, lung, liver and kidney.
DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
Post-translational modificationsIsoform 2 is palmitoylated and myristoylated while isoform 1.
Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
Cdc25 like protein antibody
Cdc25-like protein antibody
F25B3.3 kinase like protein antibody
F25B3.3 kinase-like protein antibody
RAS guanyl releasing protein 2 antibody
RAS guanyl-releasing protein 2 antibody
Anti-RASGRP2 antibody images
Western blot - RASGRP2 antibody (ab56658)
Western blot against tagged recombinant protein immunogen using ab56658 RASGRP2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-RASGRP2 antibody (ab56658)
This product has been referenced in:
Robert P et al. A novel leukocyte adhesion deficiency III variant: kindlin-3 deficiency results in integrin- and nonintegrin-related defects in different steps of leukocyte adhesion. J Immunol186:5273-83 (2011).
Read more (PubMed: 21441448) »