Overview

  • Product nameAnti-RASGRP2 antibody
    See all RASGRP2 primary antibodies
  • Description
    Mouse monoclonal to RASGRP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: GTLDLDKGCT VEELLRGCIE AFDDSGKVRD PQLVRMFLMM HPWYIPSSQL AAKLLHIYQQ SRKDNSNSLQ VKTCHLVRYW ISAFPAEFDL NPELAEQIKE , corresponding to amino acids 65-165 of Human RASGRP2

Properties

Applications

Our Abpromise guarantee covers the use of ab56658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
    • Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
    • Sequence similaritiesBelongs to the RASGRP family.
      Contains 2 EF-hand domains.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • Developmental stageExpressed in fetal brain, lung, liver and kidney.
    • DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
    • Post-translational
      modifications
      Isoform 2 is palmitoylated and myristoylated while isoform 1.
    • Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium and DAG-regulated antibody
      • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
      • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
      • CalDAG-GEFI antibody
      • Cdc25 like protein antibody
      • Cdc25-like protein antibody
      • F25B3.3 kinase like protein antibody
      • F25B3.3 kinase-like protein antibody
      • GRP2_HUMAN antibody
      • HCDC25L antibody
      • RAS guanyl releasing protein 2 antibody
      • RAS guanyl-releasing protein 2 antibody
      • Rasgrp2 antibody
      see all

    Anti-RASGRP2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56658 RASGRP2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-RASGRP2 antibody (ab56658)

    This product has been referenced in:
    • Robert P  et al. A novel leukocyte adhesion deficiency III variant: kindlin-3 deficiency results in integrin- and nonintegrin-related defects in different steps of leukocyte adhesion. J Immunol 186:5273-83 (2011). WB ; Human . Read more (PubMed: 21441448) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"