Overview

  • Product nameAnti-RASGRP2 antibody
    See all RASGRP2 primary antibodies
  • Description
    Rabbit polyclonal to RASGRP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-83 (VRMFLMMHPW YIPSSQLAAK LLHIYQQSRK DNSNSLQVKT CHLVRYWISA) of Human RASGRP2 (NP_722541)

  • Positive control
    • Human fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
  • Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
  • Sequence similaritiesBelongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • Developmental stageExpressed in fetal brain, lung, liver and kidney.
  • DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
  • Post-translational
    modifications
    Isoform 2 is palmitoylated and myristoylated while isoform 1.
  • Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium and DAG-regulated antibody
    • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
    • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
    • CalDAG-GEFI antibody
    • Cdc25 like protein antibody
    • Cdc25-like protein antibody
    • F25B3.3 kinase like protein antibody
    • F25B3.3 kinase-like protein antibody
    • GRP2_HUMAN antibody
    • HCDC25L antibody
    • RAS guanyl releasing protein 2 antibody
    • RAS guanyl-releasing protein 2 antibody
    • Rasgrp2 antibody
    see all

Anti-RASGRP2 antibody images

  • Anti-RASGRP2 antibody (ab94865) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa

References for Anti-RASGRP2 antibody (ab94865)

ab94865 has not yet been referenced specifically in any publications.

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