Anti-RASGRP2 antibody - N-terminal (ab170572)

Overview

  • Product name
    Anti-RASGRP2 antibody - N-terminal
    See all RASGRP2 primary antibodies
  • Description
    Rabbit polyclonal to RASGRP2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RASGRP2 aa 14-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q7LDG7

  • Positive control
    • Human brain tissue; U-251MG cells; K562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 69 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
  • Tissue specificity
    Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
  • Sequence similarities
    Belongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • Developmental stage
    Expressed in fetal brain, lung, liver and kidney.
  • Domain
    The N-terminal Ras-GEF domain mediates association with F-actin.
  • Post-translational
    modifications
    Isoform 2 is palmitoylated and myristoylated while isoform 1.
  • Cellular localization
    Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium and DAG-regulated antibody
    • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
    • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
    • CalDAG-GEFI antibody
    • Cdc25 like protein antibody
    • Cdc25-like protein antibody
    • F25B3.3 kinase like protein antibody
    • F25B3.3 kinase-like protein antibody
    • GRP2_HUMAN antibody
    • HCDC25L antibody
    • RAS guanyl releasing protein 2 antibody
    • RAS guanyl-releasing protein 2 antibody
    • Rasgrp2 antibody
    see all

Images

  • Immunofluorescence of U-251MG cells labeling RASGRP2 using ab170572 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling RASGRP2 with ab170572 at 1/10 dilution.

  • Anti-RASGRP2 antibody - N-terminal (ab170572) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 69 kDa

References

ab170572 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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