Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)

Overview

Applications

Our Abpromise guarantee covers the use of ab124823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionGuanine nucleotide exchange factor (GEF) for Ras and Rap1.
    • Sequence similaritiesBelongs to the RASGRP family.
      Contains 2 EF-hand domains.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
      • Grp3 antibody
      • GRP3_HUMAN antibody
      • Guanine nucleotide exchange factor for Rap1 antibody
      • Kiaa0846 antibody
      • RAS guanyl releasing protein 3 (calcium and DAG regulated) antibody
      • RAS guanyl releasing protein 3 calcium and DAG regualated guanine nucleotide exchange factor iii antibody
      • Ras guanyl-releasing protein 3 antibody
      • RASGRP3 antibody
      see all

    Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] images

    • All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution

      Lane 1 : Ramos cell lysate, untreated
      Lane 2 : Ramos cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : HRP labelled Goat anti-Rabbit at 1/2000 dilution
      Lane 2 : HRP labelled Goat anti-Rabbit at 1/2000 dilution


      Predicted band size : 78 kDa

    References for Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)

    This product has been referenced in:
    • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »
    • Song X  et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Rat . Read more (PubMed: 23991094) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"