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Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133)
ab124823 only detects RASGRP3 phosphorylated at Threonine 133.
, ICC/IFmore detailsUnsuitable for:
IHC-P or IP
Does not react with:
Phospho-specific peptide corresponding to residues surrounding Threonine 133 of Human RASGRP3.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
||1/100 - 1/250.
Is unsuitable for IHC-P or IP.
Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Information by UniProt
- Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
- Grp3 antibody
- GRP3_HUMAN antibody
Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)
All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution
Lane 1 : Ramos cell lysate, untreated
Lane 2 : Ramos cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Lane 1 : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Lane 2 : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"