Overview

  • Product nameAnti-RASSF1a antibody [114-10H1]
    See all RASSF1a primary antibodies
  • Description
    Mouse monoclonal [114-10H1] to RASSF1a
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the splice junction region of RASSF1a.

  • Positive control
    • HeLa cells extract

Properties

Applications

Our Abpromise guarantee covers the use of ab91212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 45 kDa.
IP Use at an assay dependent dilution.

Target

  • FunctionPotential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • Tissue specificityIsoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • Sequence similaritiesContains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localizationNucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras association domain containing protein 1 antibody
    • Ras association domain family 1 antibody
    • Ras association domain family protein 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • RASSF 1a antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RASSF1A isoform antibody
    • Tumor suppressor protein RDA32 antibody
    see all

References for Anti-RASSF1a antibody [114-10H1] (ab91212)

This product has been referenced in:
  • Whang YM  et al. RASSF1A suppresses the c-Jun-NH2-kinase pathway and inhibits cell cycle progression. Cancer Res 65:3682-90 (2005). WB ; Human . Read more (PubMed: 15867363) »
  • Dallol A  et al. RASSF1A interacts with microtubule-associated proteins and modulates microtubule dynamics. Cancer Res 64:4112-6 (2004). WB ; Human . Read more (PubMed: 15205320) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"