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Recombinant human RASSF1A purified from E. coli.
Our Abpromise guarantee covers the use of ab23950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
|ICC/IF||Use at an assay dependent concentration. Use at an assay dependent dilution. Use a biotinylated secondary antibody and Texas-red conjugated streptavidin. Use 4% paraformaldehyde as fixative for 20min and permeabilize in 0.4% Triton X-100.|
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|