Rat Albumin ELISA Kit (ab108789)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameRat Albumin ELISA Kit
See all Albumin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 2.9% Inter-assay Sample n Mean SD CV% Overall 9.7% -
Sample typeCell culture supernatant, Urine
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Assay typeSandwich (quantitative)
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Sensitivity= 0.4 ng/ml
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Range0.781 ng/ml - 200 ng/ml
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Recovery
97 %
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Assay time3h 00m
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Rat
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Product overview
Rat Albumin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin in cell culture supernatants and urine.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat Albumin Antibody 1 x 120µl Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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Tissue specificityPlasma.
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Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
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Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 24186 Rat
- SwissProt: P02770 Rat
- Unigene: 202968 Rat
Applications
Our Abpromise guarantee covers the use of ab108789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
Images
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Sandwich ELISA - ab108789 Albumin Rat ELISA kitRat albumin measured in tissue lysates showing quantity (microgram) per mg protein
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Sandwich ELISA - ab108789 Albumin Rat ELISA kitRat albumin measured in various samples showing quantity (microgram) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108789.
Protocols
References
This product has been referenced in:
- Sart S et al. Multiscale cytometry and regulation of 3D cell cultures on a chip. Nat Commun 8:469 (2017). Read more (PubMed: 28883466) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"