Overview

  • Product name
    Rat Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.7%
  • Sample type
    Cell culture supernatant, Urine
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.4 ng/ml
  • Range
    0.781 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Rat Albumin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin in cell culture supernatants and urine.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Rat albumin measured in tissue lysates showing quantity (microgram) per mg protein

  • Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108789.

Protocols

References

ab108789 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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