Abcam’s Albumin rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin in cell culture supernatants and urine.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Rat Albumin Antibody||1 x 120µl|
|Albumin Microplate (12 x 8 well strips)||1 unit|
|Albumin Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Rat albumin measured in tissue lysates showing quantity (microgram) per mg protein
Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample
Representative Standard Curve using ab108789.
ab108789 has not yet been referenced specifically in any publications.