Customer reviews and FAQs

Overview

  • Product name
    Rat Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.7%
  • Sample type
    Cell culture supernatant, Urine
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.4 ng/ml
  • Range
    0.781 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Albumin rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin in cell culture supernatants and urine.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108789 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent dilution.

    Rat Albumin ELISA Kit images

    • Sandwich ELISA - ab108789 Albumin Rat ELISA kit
      Sandwich ELISA - ab108789 Albumin Rat ELISA kit

      Rat albumin measured in tissue lysates showing quantity (microgram) per mg protein

    • Sandwich ELISA - ab108789 Albumin Rat ELISA kit
      Sandwich ELISA - ab108789 Albumin Rat ELISA kit

      Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108789.

    Protocols

    References for Rat Albumin ELISA Kit (ab108789)

    ab108789 has not yet been referenced specifically in any publications.

    Publishing research using ab108789? Please let us know so that we can cite the reference in this datasheet.

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